ATCC25922
Escherichia coli (Migula) CAS:tellani and Chalmers
25922™
Does not produce verotoxin. This organism is a CLSI control strain for antimicrobial susceptibility testing. It is used for media testing, as a negative control for LT toxin production, and as a quality control strain for Abbott, API, Autobac, BBL, bioMerieux Vitek, Biosynth, Difco, IDS, Micro-Media, MicroScan™, Roche Diagnostics, and Sensititre products. Used in susceptibility disc testing of neomycin, colistin [colimycin], kanamycin, cephalexin, gentamicins, cefamandole, cephalothin, tetracycline, cephaloglycin, cephaloridine [cephalomycin], nalidixic acid, and chloramphenicol.
BSL 1
ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.
ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Specific applications
susceptibility disc testing cephalexin Ref
susceptibility disc testing cephaloglycin Ref
susceptibility disc testing kanamycin Ref
susceptibility disc testing cephaloridine cephalomycin Ref Ref
Food testing
Control Culture Ref Ref Ref
control strain Ref Ref Ref
evaluation of Mueller-Hinton agar Ref Ref
media testing Ref Ref Ref Ref Ref Ref Ref
quality control Ref
quality control strain Ref Ref Ref Ref Ref Ref Ref Ref Ref Ref Ref
susceptibility disc testing Ref Ref Ref Ref
susceptibility disc testing cephalothin Ref
susceptibility disc testing chloramphenicol Ref
susceptibility disc testing colistin colimycin Ref
susceptibility disc testing gentamicins gentamicin ref
susceptibility disc testing nalidixic acid Ref
susceptibility disc testing neomycin Ref
susceptibility disc testing tetracycline Ref
susceptibility testing Ref Ref Ref Ref Ref Ref Ref Ref Ref Ref
testing Ref
quality control strain for Abbott, API, Autobac, BBL, bioMerieux Vitek, Biosynth, Difco, IDS, Micro-Media, MicroScan®, Roche Diagnostics, and Sensititre products
Preceptrol
Yes
Serotype
O6, Biotype 1
Comments
Incorrectly cited in previous editions of this catalog as identical to NCTC 10418
Biotype 1
Technical information
ATCC Technical Services does not have technical information on patent deposits that are not produced or characterized by ATCC. Additional information can be found in the corresponding patent available from the patent holder or with the U.S. and/or international patent office.
Medium
ATCC Medium 18: TryptiCAS:e Soy Agar/Broth
Temperature
37°C
Atmosphere
Aerobic
Handling procedure
-
Open vial according to enclosed instructions.
-
Using a single tube of #18 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a Pasteur or 1.0 mL pipette. Rehydrate the entire pellet.
-
Aseptically transfer this aliquot back into the broth tube. Mix well.
-
Use several drops of the suspension to inoculate a #18 agar slant and/or plate.
-
Incubate the tubes and plate at 37°C for 24 hours.
Handling notes
ATCC® 25922™ is a recommended reference strain for antibiotic susceptibility testing. It has been found that passage in broth often results in a change in MIC levels. Therefore, it is best to keep it on agar and to make stocks for storage immediately. Repeated passage is discouraged.
Purified genomic DNA of this strain is available as ATCC® 25922D-5™.
Additional information on this culture is available on the ATCC® web site at www.atcc.org.
Verification method
Whole-genome Sequencing
Deposited as
Escherichia coli (Migula) CAS:tellani and Chalmers
Depositors
FDA
Chain of custody
ATCC <-- FDA <-- F Schoenknecht
Patent depository
This material was deposited with the ATCC Patent Depository to fulfill U.S. or international patent requirements. This material may not have been produced or characterized by ATCC. As an International Depository Authority (IDA) for patent deposits, ATCC is required to complete viability testing only at time of initial deposit of patent material. Patent deposits are made available on behalf of the Depositor when the pertinent U.S. or international patent is issued, but material may not be used to infringe the patent claims.
Patent number
7,291,480
Cross references
GenBank AX110212 Sequence 945 from Patent WO0123604.
GenBank AX110242 Sequence 975 from Patent WO0123604.
GenBank AX111547 Sequence 2280 from Patent WO0123604.
GenBank X80724 E. coli (ATCC 25922) gene for 16S rRNA.
GenBank AF038431 Escherichia coli DNA gyrase A (gyrA) gene, partial cds.
Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
